The THRIFTY method is based on small, short segments of the muscle fibers separated from the original fibers and then mounted on a specially adapted glass slide. The glass slide is then stained with established antibody technology against specific proteins in the structure of the fibers. By illuminating the segment with fluorescent light in a microscope, one can quickly determine whether the element belongs to a slow or fast muscle fiber. The method is so time-efficient that it allows fiber-type determination of living muscle fibers.
– The study is of great value to us as we have several new projects that build on this methodology. We are convinced that the method will contribute to many further exciting research questions being answered in the future, says responsible researcher William Apró.
It is researcher William Apró, PhD, who, with doctoral students Oscar Horwath and Sebastian Edman, has recently published the new study in The Journal of Physiology.